Research related to Chemistry & Biology

Methods and research techniques have been studied and developed to look specifically at Sainfoin polyphenols which include tannins and flavonoids. As anticipated there were significant differences between Sainfoin types and this will lead to further development of Sainfoin plant breeding.

Scientific Articles/Findings

Acylated flavonol glycosides from the forage legume, Onobrychis viciifolia (sainfoin). Phytochemistry, 2011, 72, 423-429.

Veitch N.C., Regos I., Kite . G.C., Treutter D.

Binding of pentagalloyl glucose to two globular proteins occurs via multiple surface sites

University of Reading - Dobreva, M.A., Frazier, R.A., Mueller-Harvey, I., Clifton, L.A., Gea, A., Green, R.J. Biomacromolecules 2011, 12, 710-715.

Binding of pentagalloyl glucose to two globular proteins occurs via multiple surface sites. Biomacromolecules 2011, 12, 710-715.

Dobreva, M.A., Frazier, R.A., Mueller-Harvey, I., Clifton, L.A., Gea A., Green, R.J.

Biosynthesis of flavonoids in Sainfoin

Technical University of Vienna - Asma F. Ahmad, Jana Thill, Ionela Regos, Christine Hayot, Heidi Halbwirth, Lydia Smith, Dieter Treutter, Karl Stich

Characterisation of sainfoin (Onobrychis viciifolia) condensed tannins by MALDI-TOF mass spectrometry

Reading University - Elisabetta Stringano, An Gea, Rainer K. Cramer, Wayne Hayes & Irene Mueller-Harvey

Characterisation of Sainfoin tannins by degradation studies chromatography and calorimetry

Reading University - An Gea, Elisabetta Stringano, Marina A. Dobreva, Trevor Gibson, Richard A. Frazier, Rebecca J. Green & Irene Mueller- Harvey

Deciphering the complexity of sainfoin (Onobrychis viciifolia) proanthocyanidins by MALDI-TOF mass spectrometry with a judicious choice of isotope patterns and matrices. Anal. Chem. 2011, 83, 4147-4153

Stringano, E., Cramer, R., Hayes, W., Smith, C., Gibson, T., Mueller-Harvey, I.

Deciphering the complexity of sainfoin (Onobrychis viciifolia) proanthocyanidins by MALDI-TOF mass spectrometry with a judicious choice of isotope patterns and matrices. Analytical Chemistry 2011, 83, 4147-4153.

Reading University - Stringano E., Cramer R., Hayes W., Smith, C., Gibson T., Mueller-Harvey I.

Effect of different conservation methods and maturity stages on peroxidase activity in sainfoin (Onobrychis viciifolia)

Technical University of Vienna - Asma Faqir Ahmad, Katerina Theodoridou, Heidi Halbwirth, Jocelyne Aufrčre, Karl Stich

Effect of wilting, silage additive, PEG treatment and tannin content on the distribution of N between different fractions after ensiling of three different sainfoin (Onobrychis viciifolia) varieties. Grass and Forage Science, 65, 175-184.

Lorenz, M.M., Eriksson, T., Uden, P. 2010

Exploring variation in proanthocyanidin composition and content of sainfoin (Onobrychis viciifolia). J. Sci. Food Agric. 2013, 93, 2102-2109.

Azuhnwi, B.N., Boller, B., Dohme-Meier, F., Hess, H.D., Kreuzer, M., Stringano, E., Mueller-Harvey, I.

Flavonoids and their formation in different sainfoin varieties (Onobrychis viciifolia)

Technical University of Vienna & Technische Universitat, Munchen - Jana Thill, Ionela Regos, Christine Hayot, Ilya Gadjev, Karin Schlangen, Heidi Halbwirth, Dieter Treutter, Lydia Smith, Karl Stich

Identification and quantification of phenolic compounds from the forage legume sainfoin (Onobrychis viciifolia). J. Agric. Food Chem. 2009, 57, 5843-5852.

Regos I., Urbanella A., Treutter D.

In situ analysis and structural elucidation of sainfoin (Onobrychis viciifolia) tannins for high throughput germplasm screening. J. Agric. Food Chem. 2011, 59, 495-503.

Gea, A., Stringano, E., Brown, R.H., Mueller-Harvey, I.

In vitro protein degradation of 38 sainfoin accessions and its relationship to tannin content by different assays. J. Agric. Food Chem. 60, 5071−5075

Lorenz, M.M., Hayot Carbonero, C., Smith, L., Udén, P., 2012

Influence of formic acid and dry matter on protein degradation in the tanniniferous legume sainfoin. Animal Feed Sci Technol. 164, 217–224.

Lorenz, M.M., Udén, P., 2011

Investigation of sainfoin (Onobrychis viciifolia) cultivar differences on nitrogen balance and fecal egg count in artificially infected lambs. J. Anim. Sci. 2013, 91, 2342-2354.

Azuhnwi, BN, Hertzberg, H, Arrigo, Y, Gutzwiller, A, Hess, HD, Mueller-Harvey, I., Torgerson, P, Kreuzer, M, Dohme-Meier, F

Morphology, tannin concentration and forage value of 15 Swiss accessions of sainfoin (Onobrychis viciifolia Scop) as influenced by harvest time and cultivation site. Grass Forage Sci. 2011, 66, 474-487.

Azuhnwi, B.N., Boller, B., Martens, M., Dohme-Meier, F., Ampuero S., Gunter, S., Kreuzer, M., Hess, H.D.,

Near Infrared Spectroscopy (NIRS) for Screening Sainfoin Germplasm

Reading University - Martin-Lorenzo M, Hayot C, Stringano E, Falco-Sales L, Smith L, Barnes R, Oliver J, Brown R H, Mueller-Harvey I

Optimization of a high-performance liquid chromatography method for the analysis of complex polyphenol mixtures and application for sainfoin extracts (Onobrychis viciifolia). J. Chromatogr A 1217, 6169-77.

Regos I., Treutter D. 2010

Phylogenetic characterisation of Onobrychis species with special focus on the forage crop Onobrychis viciifolia Scop.

Reading University & NIAB - Hayot Carbonero Christine, Carbonero Franck, Smith Lydia, Brown Terence

Polyphenol metabolism provides a screening tool for beneficial effects of Onobrychis viciifolia (sainfoin). Phytochemistry. 82, 67-80

Thill J., Regos I., Farag M.A., Ahmad A.F., Kusek J., Castro A., Schlangen K., Carbonero C.H., Gadjey I.Z., Smith L.M., Halbwirth H., Treutter D., Stich K. 2012.

Proanthocyanidin diversity in the EU ‘HealthyHay’ sainfoin (Onobrychis viciifolia) germplasm collection

University of Reading and NIAB - Stringano, E., Hayot Carbonero, C, Smith, L, Brown, RH, Mueller-Harvey, I. Phytochemistry (doi: 10.1016/j.phytochem.2012.01.013)

Proanthocyanidin diversity in the EU ‘HealthyHay’ sainfoin (Onobrychis viciifolia) germplasm collection Phytochemistry 2012, 77, 197-208.

Stringano, E., Hayot Carbonero, C., Smith, L.M.J., Brown, R.H., Mueller-Harvey, I.

Ruminal dry matter and crude protein degradation kinetics of five sainfoin (Onobrychis viciifolia Scop) accessions differing in condensed tannin content and obtained from different harvest. Anim. Feed Sci. Tech. 2012, 177, 135-143.

Simple solution for a complex problem: proanthocyanidins, galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography.

University of Reading and University of Turku, Finland - Stringano, E., Gea, A., Salminen, J-P, Mueller-Harvey, I. J. Chromatogr. A 2011, 1218, 7804-7812.

Simple solution for a complex problem: proanthocyanidins, galloyl glucoses and ellagitannins fit on a single calibration curve in high performance-gel permeation chromatography. J. Chromatogr. A 2011, 1218, 7804-7812.

Stringano, E., Gea, A., Salminen, J-P., Mueller-Harvey, I.

Size and molecular flexibility affect the binding of ellagitannins to bovine serum albumin. J. Agric. Food Chem. 2014, 62, 9186-9194.

Dobreva M.A., Green R.J., Mueller-Harvey I., Salminen J-P., Howlin B.J., Frazier R.A.

Tannins Explained

Reading University - Gea, A., Stringano, E., Brown, R.H., Mueller‐Harvey, I. In situ analysis and structural elucidation of sainfoin (Onobrychis viciifolia) tannins for high throughput germplasm screening. J. Agric. Food Chem. 2011, 59, 495‐503.

The effects of condensed tannins against cattle nematodes (oral presentation). 23rd International Conference of the World Association for the Advancement of Veterinary Parasitology Congress, 21 to 25 August 2011, Buenos Aires, Argentina.

Swedish University of Agricultural Sciences, University of Copenhagen, University of Reading - Novobilský, A., Thamsborg, S.M., Mueller-Harvey, I.

Towards Developing Plants for Production of Useful Tannins

Univerity of Reading - I. Mueller-Harvey, E. Stringano, L. Falchero, C. Hayot-Carbonero, L. Smith, S. Hanley, I. Shield, A. Karp